We sequence different kinds of cloned DNA
as well as PCR-fragments!
We provide solutions for more problematic
templates as well!
(see quality)
We are glad to choose suitable primers for
your sequencing projects!
(see primer data base)
Reaction conditions are individually adjusted for every sample and each sequence is edited manually.
We also provide unedited raw data ("non-edit").
Reading lengths provided are:
Service per reaction includes (without additional costs):
1. Quality and quantity check of DNA samples
We check the quality and quantity of your sample on agarose gels.
2. Purification and concentration of DNA samples
Samples, with low amount or poor quality of DNA, are purified (ethanol precipitation) and concentrated. If the amount of DNA is too low, we will contact you beforehand and discuss further options.
3. Primer check
We check your primers regarding vector specifity and suggest appropriate alternatives in case. We also choose primers for you according to reference sequences and provided vector information. If possible, we use one of 1000 primers in stock, to reduce costs and time.
Primer data base
Primer data base
4. Individual adjustment of reaction conditions
Reaction conditions are adjusted individually (depending on amount of sample DNA, annealing temperature, sequencing protocols etc.) according to our long-standing experience in this field.
5. Repeated sequencing reactions
We track down the reasons for unsatisfying results or failed reactions (for example signal drop due to secondary structures, low signal intensity, major background signals) and repeat reactions under relevant adjustments without any further costs.
6. Editing of raw data
We edit raw data completely and thoroughly, which includes editing of problematic raw data like sequences with strong background signals and frameshifts (see quality).
We additionally quantify any polymorphisms.
We additionally quantify any polymorphisms.
7. Presentation of results, assembly of contigs and comparison with reference sequences
Edited sequences are combined into alignments and sent to you as pdf-files. We assemble overlapping sequences into contigs and compare your results with provided references.
8. Detailed comments on your results
Our specialized staff add detailed comments on data reliability, possible reasons for unsatisfying results and advice on further or alternative proceedings.
9. Optional shipment of data
Data are forwarded as sequence files (txt, fasta), electropherograms (ab1, scf), alignments (pdf), Vector-NTI-archive (ma4) either electronically (email or online-order-system) or as hardcopy print-outs, all according to your preferences.
10. Free storage
Your samples and primers are stored and remain accessible for one year. We also archive your data for at least one year.
For a few services we charge an additional fee:
1. Column purification
For removing amplification primers from PCR products.
2. Gel extraction
Specific PCR bands are extracted from agarose gels and purified.
3. Plasmid-DNA-preparation from bacterial pellets
4. Direct sequencing of bacterial pellets
max. reading length 780bp!
